Nucleotide and Amino Acid Changes in HN, F and SH genes of an Iranian Mumps Virus; RS-12, Following Attenuation to Vaccine Strain
Authors
Abstract:
Background and Aims: Wild-type RS-12 strain of mumps virus has been isolated from an Iranian patient and has been attenuated after several serial passages. This study was designed to determine nucleotide and amino acid substitutions in the HN, F and SH genes during attenuation of the wild-type virus. Materials and Methods: Required viral samples prepared at Razi Vaccine and Serum Institute. Viral RNA was extracted, targeted segments were amplified and sequenced and finally were analyzed using DNAMAN software. Results: No difference in nucleotide and deduced amino acid sequence of the F and SH genes was detected following attenuation of wild-type as compared to the vaccine strain, but four nucleotide changes in HN gene had occurred which had resulted in two nucleotide differences. Conclusions: Considering the HN nucleotide sequences and the deduced amino acid sequences, RS-12 wild-type and vaccine strains were distinguishable. Moreover, unique differences between RS-12 and some other vaccine strains existed. During serial passages of RS-12 strain for seed lot system preparation, no change in HN, F and SH genes occurred which -at least in some extents- proved the genetic stability of the attenuated RS-12 surface proteins. Potential attenuating mutations in other genes (N, P, M, L and even non-coding regions in 3′ and 5′ ends) should be investigated and confirmed using advanced methods
similar resources
Stability Study of Measles AIK-C Strain, Mumps RS-12 Strain and Rubella Takahashi Strain in MMR Vaccine
Stability studies play a critical role in assuring product quality at all points in the vaccine life cycle and havea major impact on the success of immunization programs worldwide. The purpose of stability study isdetermination of the vaccine quality under the variety of environmental factors and to establish a re-testperiod or a shelf life and recommended storage conditions. In this study thre...
full textstability study of measles aik-c strain, mumps rs-12 strain and rubella takahashi strain in mmr vaccine
stability studies play a critical role in assuring product quality at all points in the vaccine life cycle and havea major impact on the success of immunization programs worldwide. the purpose of stability study isdetermination of the vaccine quality under the variety of environmental factors and to establish a re-testperiod or a shelf life and recommended storage conditions. in this study thre...
full textEstablishment of a standard seed lot system of an Iranian Mumps virus strain; RS-12, for mass production of mumps and MMR vaccines
BACKGROUND At present the mumps virus strain used for production of mumps vaccine for our local use is Hoshino strain. However, according to our National public health policies, this strain should be replaced with a safer strain. Based on our previous data, the Iranian mumps strain; RS-12 has been proved to be the most suitable alternative to Hoshino strain with little or no adverse events foll...
full textan investigation into iranian teachers consistency and bias in evaluation of students writings
while performance-based language assessment has led to an increased authenticity and content validity in the practice of writing assessment, the reliability of ratings has become a major issue. research findings have shown different reactions by native english speaker (nes) and non-native english speaker (nns) teachers to students’ writings. the focus of this study is on investigating whether i...
My Resources
Journal title
volume 10 issue None
pages 26- 33
publication date 2016-12
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023